Reyes De Los Santos, Aníbal Marcelo

Research interests

During my undergraduate and graduate studies, I gained experience in investigating the detoxification of oxidant molecules through thiol-dependent peroxidases (peroxiredoxins, Prx). In particular, I participated in the enzymatic and physicochemical characterization of two Prx from Mycobacterium tuberculosis, the causative agent of tuberculosis. One of them was used as a model to implement an interdisciplinary approach that combined experimental (bench-based) and in silico techniques, with the aim of addressing general questions regarding the catalysis of this type of antioxidant enzymes. The analysis of the second Prx was focused on evaluating the importance of its activity for pathogen viability under conditions in which cholesterol is the sole carbon source.

In parallel with these studies, I acquired knowledge on the formation of reactive oxygen and nitrogen species, both in physiological contexts—such as during macrophage infection—and at basal levels (primarily in the mitochondria). This led me to enzymatically characterize a third, human peroxiredoxin (Prx3), recognized as one of the main antioxidant systems of this organelle.

This experience contributed to the preparation of several scientific articles, research projects, and the supervision of an undergraduate student. Currently, I work in the mass spectrometry unit (CEINBIO-PAyS), where I develop both targeted and untargeted strategies for the detection and quantification of small molecules in different matrices, including natural extracts and biological samples from individuals involved in various studies. This stage represents a significant challenge and finds me actively engaged in training in sample preparation, HPLC-MS method development, and data processing and interpretation.